Coding
crnA

Part:BBa_K5108004

Designed by: Léa Breton   Group: iGEM24_Toulouse-INSA-UPS   (2024-08-28)

Creatinine amidohydrolase form Pseudomonas putida

Creatinine amidohydrolase from Pseudomonas putida


    Contents
  1. Usage and Biology
  2. Characterization and Measurements
  3. References



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 61
    Illegal NgoMIV site found at 663
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 199
    Illegal BsaI.rc site found at 547

Usage and Biology

Creatinine amidohydrolase (CrnA) is an enzyme that catalyzes the degradation of creatinine into creatine in Pseudomonas putida. In the context of our project, the gene encoding this enzyme (crnA) was synthesized in an operon together with the gene for creatine degradation. You can find more informations about construct and results in BBa_K5108009.


Characterization and Measurements

SDS-PAGE, growth analysis and consumption analysis of creatinine by NMR spectroscopy were performed. The results can be found in BBa_K5108009 and in iGEM ToulouseINSA-UPS 2024.


References

  • UniProt. (s. d.). https://www.uniprot.org/uniprotkb/P83772/entry
  • Tsuru, D., Oka, I., & Yoshimoto, T. (1976c). Creatinine Decomposing Enzymes inPseudomonas putida. Agricultural And Biological Chemistry, 40(5), 1011‑1018. https://doi.org/10.1080/00021369.1976.10862151
  • [edit]
    Categories
    //cds/enzyme
    Parameters
    biologyPseudomonas putida
    proteinCreatinine amidohydrolase
    uniprotP83772